Chizuru Yukimura (雪村 千鶴 Yukimura Chizuru) : Houko Kuwashima (Japanese), Brittney Karbowski (English)
Toshizo Hijikata (土方 歳三 Hijikata Toshizō): Shinichiro Miki (Japanese), Andrew Love (English)
Soji Okita (沖田 総司 Okita Sōji): Showtaro Morikubo (Japanese), Blake Shepard (English)
Isami Kondo (近藤 勇 Kondō Isami?) Voiced by: Tōru Ōkawa (Japanese), David Wald (English)
Keisuke Yamanami (山南 敬助 Yamanami Keisuke): Nobuo Tobita (Japanese), David Matranga (English)
Shinpachi Nagakura (永倉 新八 Nagakura Shinpachi): Tomohiro Tsuboi (Japanese), Kalob Martinez (English)
Sanosuke Harada (原田 左之助 Harada Sanosuke): Kōji Yusa (Japanese), Illich Guardiola (English)
Heisuke Todo (藤堂 平助 Tōdō Heisuke): Hiroyuki Yoshino (Japanese), Greg Ayres (English)
Hajime Saito (斎藤 一 Saitō Hajime): Kōsuke Toriumi (Japanese), Leraldo Anzaldua (English)
Kaoru Nagumo (南雲 薫 Nagumo Kaoru): Hasumi Itō
Chikage Kazama (風間 千景 Kazama Chikage): Kenjirō Tsuda (Japanese), Corey Hatzog (English)
Kyuujyu Amagiri (天霧 九寿 Amagiri Kyūju?): Ryū Yamaguchi (Japanese), Steven Fenley (English)
Kyo Shiranui (不知火 匡 Shiranui Kyō?): Hiroaki Yoshida (Japanese), Houston Hayes (English)
Sen-hime (千姫?) : Ayano Ishikawa (Japanese), Hilary Haag (English)